Development of Validated Spectrofluorimetric Method for the Estimation of Fenoverine

نویسندگان

  • A. Suganthi
  • M. Srikanth
  • N. Ramu
چکیده

For the estimation of fenoverine in pure and pharmaceutical preparation a sensitive spectrofluorimetric method was developed. This wass based on the oxidation of fenoverine with cerric ammonium sulphate to produce cerrous ion, whose fluorescence was observed at 376 nm while excited at 261 nm. The calibration graph was linear over the range of 1-10 μg/ml. This is applied successfully for the assay of fenoverine in marketed formulations with a mean value of 98.70 ± 0.5219. Recoveries were found to be 99.14% and 99.42 % at 50 and 100% level with the % RSD values of 0.432% and 0.673%, respectively.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Development of a Sensitive Spectrofluorometric-Multivariate Calibration Method for Enzyme Kinetic of Aldehyde Oxidase

Attempts to obtain experimental values for the kinetic parameters of phenanthridine oxidation by guinea pig or rabbit liver aldehyde oxidase using common spectrophotometric methods have not been successful due to a lower limit of detection. In the present study, a new spectrofluorimetric assay in combination with a multivariate calibration method for enzymatic kinetic study of aldehyde oxidase ...

متن کامل

Development of a Sensitive Spectrofluorometric-Multivariate Calibration Method for Enzyme Kinetic of Aldehyde Oxidase

Attempts to obtain experimental values for the kinetic parameters of phenanthridine oxidation by guinea pig or rabbit liver aldehyde oxidase using common spectrophotometric methods have not been successful due to a lower limit of detection. In the present study, a new spectrofluorimetric assay in combination with a multivariate calibration method for enzymatic kinetic study of aldehyde oxidase ...

متن کامل

Spectrofluorimetric Estimation of Scopoletin in Evolvulus alsinoides Linn. and Convulvulus pluricaulis Choisy.

A simple sensitive spectrofluorimetric method was developed for the analysis of total coumarins calculated as scopoletin in the plants Evolvulus alsinoides and Convulvulus pluricaulis. The fluorescent nature of scopoletin proved to be of immense value in the development of the spectrofluorimetric method. The excitation and emission wavelengths for scopoletin were 430 nm and 460 nm respectively ...

متن کامل

Development of Spectrofluorimetric and HPLC Methods for In vitro Analysis of Repaglinide

Spectrofluorimetric and high-performance liquid chromatography methods for estimation of repaglinide were developed. These methods were validated for estimation of repaglinide in tablets as well as in receptor fluid obtained during in vitro permeation studies. Repaglinide was observed to exhibit emission and excitation wavelengths, respectively, at 379 nm and 282 nm with linearity in the concen...

متن کامل

Development and Validation of Bioanalytical Method for Simultaneous Estimation of Nebivolol Enantiomers in Human Plasma Using Liquid Chromatography-tandem Mass Spectrometry

The present study describes a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous determination of S-RRR and R-SSS nebivolol (nebivolol enantiomers) in human plasma using solid phase extraction technique. Method of both S-RRR and R-SSS nebivolol (nebivolol enantiomers) has been developed and validated using racemic nebivolol D4 as an internal standard. Analytes...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2013